地塞米松对HepG2细胞分泌载脂蛋白的影响

摘要

以培养的人肝癌细胞系HepG2为研究对象，采用“冻干浓缩培养液载脂蛋白测定法”，考察了地塞米松对rnHepG2细胞载脂蛋白(apolipoprotein，apo)AI、AⅡ、CⅢ、B100及E分泌的影响.结果表明：地塞米松对rnHepG2细胞apoAⅠ和apoE的分泌有促进作用，对apoAⅡ、apoB100和apoCⅢ的分泌有抑制作用，且这种作用随rn地塞米松浓度的增加而增强.当培养液中地塞米松的浓度为5.5×10-5mol/L时，apoAⅠ和apoE的分泌分别增rn加36.6％和49.4％(P＜0.01)，apoAⅡ、apoB100和apoCⅢ的分泌分别减少38.9％、31.9％和29.8％(P＜rn0.01).%In order to obersve the effect of dexamethasone on the secretion of apolipoproteins A Ⅰ, A Ⅱ, C Ⅲ,rnB100 and E by cultured HepG2 cells. The apolipoprotiens contents in culture media were measured byrnradioimmuodiffusion assay (RID) kits developed by authors' research unit. 20-fold lyophilizely condensed culturernmedia were used for the assays. The results showed that dexamethasone can increase the secretion of apoC andrnE, and inhibit the secretion of apoAⅡ , B100 and CⅢ; and the effect of dexamethasone were strengthened in arndose-dependent manner. When the concentration of dexamethasone was 5.5 × 10-5mol/L in the culture media,rnthe secretion of apolipoprotein A Ⅰ and E increased 36.6% and 49.4% (P ＜ 0.01) respectively, while thernsecretion of apo AⅡ , B100 and CⅢ decreased 38.9%、 31.9% and 29.8% (P＜0.01) respectively.