Objective To investigate the absorption kinetics of lomefloxacin at different intestine segments in rats. Methods The absorption kinetics was investigated using situ perfusion method in rats. UV and HPLC were used to determine the concentrations of phenol sulfonphthalein and Iomefloxacin, respectively. Results The absorption rate constants(Ka)at duodenum,jejunum,ileum and colon were 0. 204 5,0. 321 0,0. 183 9,0. 129 2 h-1 respectively;Ka from intestine at lomefloxacin concentration of 1.5,15,150 μg/mL were 0. 192 7,0. 219 2,0. 230 9 h -1; Ka at pH of 6. 29,6. 79 and 7.4 were 0. 163 1,0. 181 9,0. 160 2 h -1 ,respectively. Conclusion Lomefloxacin hydrochloride in rat intestines are well absorbed, absorption consistent with a kinetic characteristics of absorption of passive transport mechanism.%目的 研究盐酸洛美沙星在大鼠各肠段吸收的药代动力学特征.方法 采用大鼠在体肠段灌流实验装置,利用紫外分光光度法和HPLC分别测定酚红和盐酸洛美沙星的含量.结果 盐酸洛美沙星在十二指肠、空肠、回肠、结肠的吸收速率常数分别为0.204 5、0.321 0、0.183 9、0.129 2 h-1;在不同药物浓度1.5、15、150 μg/mL小肠的吸收速率常数分别为0.192 7、0.219 2、0.230 9 h-1;在不同pH值(6.29、6.79和7.4)时的吸收速率为0.163 1、0.181 9、0.160 2 h-1.结论 盐酸洛美沙星在大鼠全肠段均有吸收,吸收符合一级动力学特征,吸收机制为被动转运.
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