药物AB0315大鼠在体肠吸收中的动力学研究

摘要

目的 研究药物AB0315在大鼠各肠段吸收的药代动力学特征.方法 采用大鼠在体肠段灌流实验装置,利用高效液相色谱法和紫外分光光度法分别测定肠循环液中药物AB0315和酚红的含量.结果 药物AB0315在十二指肠、空肠、回肠、结肠的吸收速率常数分别为0.790 2、0.625 3、0.681 2、0.832 4 h-1;在不同药物浓度40、80、250 μg/mL小肠的吸收速率常数分别为0.575 4、0.719 1、0.845 5 h-1;在不同pH值(4.0、6.5、7.4)时的吸收速率常数分别为0.742 2、0.612 7、0.562 8 h-1.结论 药物AB0315在大鼠小肠全肠段均有吸收,并且药物的吸收呈一级动力学,吸收机制为被动转运.%Objective To investigate the pharmacokinetic characteristics of AB0315 in absorption at different intestine segments in rats. Methods The absorption kinetics was investigated by using situ perfusion method in rats. HPLC and UV were used to determine the concentrations of AB0315 and phenol sulfonphthalein, respectively. Results The absorption rate constants (Ka) at duodenum, jejunum, ileum and colon were 0.790 2, 0.625 3, 0.681 2, 0.832 4 h-1, respectively; Ka from intestine at AB0315 concentration of 40, 80, 250 μg/mL were 0.575 4, 0.719 1, 0.845 5 h-1; Ka at pH of 4.0, 6.5 and 7.4 were 0.742 2, 0.612 7, 0.562 8 h-1, respectively. Conclusion AB0315 in rat intestines are well absorbed, absorption consistent is a kinetic characteristics with absorption of passive transport mechanism.