三七总皂苷对大鼠腹部游离皮瓣存活的影响

摘要

目的观察三七总皂苷对大鼠腹部游离皮瓣氧化应激、血液流变学以及皮瓣存活率的影响,初步探讨三七总皂苷影响皮瓣存活的可能机制。方法健康清洁雄性SD大鼠144只,随机平均分为假手术组( SO组),缺血再灌注组( IR组)和三七总皂苷干预组( PNS组)。建立大鼠右下腹部游离皮瓣模型。在术前30 min分别给予生理盐水( SO组、IR组)、三七总皂苷注射液( PNS组)腹腔注射,一次性给药。术后检测各组大鼠血液中丙二醛( MDA)含量、超氧化物歧化酶( SOD)活性及血液流变学指标,并观察皮瓣成活率。结果 IR组、PNS组于皮瓣组织再灌注后(即血管吻合成功后)4、8、24 h 3个时点,MDA含量均高于SO组,SOD活性及血液流变学指标则低于SO组(P<0．05)；同时,PNS组在这3个时点的各项指标又优于IR组(P<0．05)。皮瓣成活率为：缺血再灌注组<三七总皂苷组<假手术组(P<0．01)。结论三七总皂苷能通过改善微循环和提高机体抗氧化的能力,促进皮瓣成活。%Objective To investigate the effects of panax notoginseng saponins ( PNS) on hemorrheology and oxidative stress in rats,and to explore the protective effects and mechanism of PNS on survival of free abdominal skin flap. Methods 144 male SD rats were classified into sham operation group ( SO group) ,ischemia/reperfusion group ( IR group) and PNS intervening group ( PNS group) at random. To establish the models of free skin flap in rat right abdomen. 30 min before surgery,rats were intraperitoneal injected PNS for PNS group and saline for SO and IR group. The level of malonyldialdehyde ( MDA) and the superoxide dismutase ( SOD) activity were compared between these groups,and the survival rate of flap was observed using image analysis. Results The expression of MDA in IR and PNS group was higher than that in the SO group at 4, 8, 24 hours after reperfusion, especially in the IR group (P<0. 05). The activity of SOD and hemorrheology in IR and PNS group was lower than that in the SO group at 4,8, 24 hours after reperfusion,especially in the IR group (P<0. 05). The survival rate of flap was:IR group