三羟基苯甲酸诱导HL-60细胞凋亡的实验性研究

摘要

目的 观察3,4,5-三羟基苯甲酸对HL-60细胞的增殖抑制及诱导其凋亡的作用. 方法 MTT法检测3.125、6.25、12.5、25、50、100μg/mL TBA对HL-60细胞生长的抑制作用,吖啶橙荧光染色、流式细胞仪检测细胞凋亡;结果TBA显著抑制HL-60细胞的增殖,其作用呈剂量依赖性. 18.7μg/mL TBA作用HL-60细胞48小时后AO染色,荧光显微镜下可见细胞出现体积缩小,核碎裂、染色质凝集等凋亡形态改变,HL-60细胞凋亡率为(28.24±5.24)%、与对照组(2.53±0.56)%比较差异有显著性意义(P＜0.05). 结论 TBA可能通过诱导HL-60细胞凋亡抑制其生长.%Objective To investigate the inhibition and apoptotic effect of trihydroxybenzoic acid on HL - 60 cells. Methods MTT - based cytotoxicity assay was used to test the HL - 60 cells proliferation. The apoptotic: cells were detected by AO fluoresc:ent staining and flow cytometry; Results TBA inhibited HL - 60 cells proliferation in a dose - dependent manner, HL - 60 cells treated with TBA for 48 h.AO fluorescent staining results showed that part of cell cytoplasm condensed, the volume was narrow. nuclear shrinkage, chromatin increased, more brightly fluorescent, condensed to form dense green fluorescent apoptotic features such as. Compared with control group( 2.53±0.56 )％ , the percentage of early apoptotic cells was( 28.24±5.24 )％ .the difference was significant( P ＜ 0.05 ). Conclusion TBA inhibited the proliferation of HL - 60 cells by inducing apoptosis.