AEG-1基因促进乳腺癌细胞株MCF-7转移

摘要

Objective The objective of this study was to observe the effect of AEG-1 gene on the metastasis in breast cancer MCF-7 cells.Methods AEG-1 siRNAs were transfected into MCF-7 cells to silence AEG-1 expression,and negative siRNA was used as a control.Transwell chamber was used to detect the ability of cell migration and invasion of MCF-7 cells.CCK8 assay was used to detect the cell proliferation of MCF-7 cells.At the same time,the effect of VEGF on the angiogenesis was investigated by detecting the changes of lumen formation in HUVEC cells.Results The migration,invasive and proliferative abilities were significantly inhibited in MCF-7 cells transfected with AEG-1 siRNA.Knockdown AEG-1 was significantly decreased the level of VEGF in the supernatant of MCF-7 cells.Knockdown AEG-1 was also significantly inhibited the angiogenesis activity in HUVEC cells.Conclusion Knockdown AEG-1 can significantly inhibit the migration of MCF-7 cells,including cell migration,invasion,proliferation and angiogenesis.These results suggest that AEG-1 plays an important role in the metastasis process of breast cancer and opens up new ideas for future treatment breast cancer.%目的 观察AEG-1基因在细胞水平对乳腺癌细胞MCF-7转移的影响.方法 通过将siRNA转染进MCF-7细胞,沉默细胞中AEG-1表达量,以转染阴性siRNA作为对照组.分别采用Transwell小室检测细胞迁移侵袭能力、CCK8实验检测细胞增殖能力.同时通过检测细胞中VEGF的变化及HUVEC细胞体外管腔形成实验考察AEG-1对于血管新生的影响.结果 沉默AEG-1,MCF-7细胞的迁移能力、侵袭能力和增殖能力明显受到抑制.沉默AEG-1,MCF-7细胞的VEGF表达明显降低.上清处理HUVEC细胞,沉默AEG-1组的血管新生能力明显受到抑制.结论 沉默AEG-1基因能显著抑制MCF-7细胞转移的多个层面,包括细胞迁移、侵袭、增殖以及血管新生.表明AEG-1基因在乳腺癌转移过程中起着重要作用,也为将来乳腺癌治疗开拓了新思路.