冻融卵裂期胚胎移植中过夜培养对临床妊娠结局的影响

摘要

Objective To explore the impact of culture overnight during frozen -thawed cleavage-stage embryo transfers on pregnancy outcome.Methods Data of 237 cycles of vitrified cleavage-stage embryos from the Third Affiliated Hospital between De -cember 2011 and December 2012 was retrospectively analyzed.The embryo were divided into group A (embryo was thawed on the day of transplantation) and group B (embryo was culture overnight ).The embryo implantation rate,clinical pregnancy rate and abortion rate were compared between the two groups .Results The embryo implantation rate was significantly lower in the group A when compared with that in the group B (P <0.05).However,no significant difference in clinical pregnancy rate and abortion rate was found between the two groups.Conclusion It may provide a valuable reference for the selection of high quality of embryo when embryos are thawed in advance and cultured overnight ,and then the development of embryos can be observed .This method also facilitates the arrangement of laboratory working.%目的：探讨冻融卵裂期胚胎移植中过夜培养对临床妊娠结局的影响。方法2011年12月至2012年12月在我院生殖医疗中心接受玻璃化冷冻的卵裂期胚胎移植患者共237个周期，其中胚胎解冻时间为移植当日128个周期（A 组），过夜培养109个周期（B 组），比较两组的胚胎种植率、临床妊娠率、流产率。结果 A 组胚胎种植率低于 B 组（P ＜0．05），而临床妊娠率和流产率与 B 组，差异无统计学意义（P ＞0．05）。结论将胚胎提前解冻培养过夜，观察解冻后胚胎的进一步发育情况，可提高胚胎种植率，为临床选择移植胚胎提供有意义的参考。