非小细胞肺癌患者核苷二磷酸激酶1和表皮生长因子受体表达情况及其临床意义

摘要

目的：分析非小细胞肺癌（NSCLC）患者核苷二磷酸激酶1（nm23）、表皮生长因子受体（EGFR）表达情况及其临床意义。方法选取2010年2月—2014年2月潍坊市妇幼保健院和潍坊市人民医院收治的住院 NSCLC患者72例作为观察组，同期在潍坊市妇幼保健院和潍坊市人民医院行肺大泡切除术者25例作为对照组，收集观察组患者术中切除的癌组织标本及对照组患者术中切除的正常肺组织标本。采用免疫组织化学染色法检测 nm23、EGFR 表达情况。结果癌组织标本中 nm23阳性表达率为66.7%，低于正常肺组织标本的84.0%（P ﹤0.05）；癌组织标本中EGFR 阳性表达率为58.3%，高于正常肺组织标本的16.0%（P ﹤0.01）。不同性别、年龄、病理类型、肿瘤直径、分化程度、TNM 分期及有无吸烟史 NSCLC 患者 nm23、EGFR 阳性表达率比较，差异无统计学意义（P ﹥0.05）；有无淋巴结转移 NSCLC 患者 nm23阳性表达率比较，差异有统计学意义（P ﹤0.01）；而有无淋巴结转移 NSCLC 患者 EGFR 阳性表达率比较，差异无统计学意义（P ﹥0.05）。Spearman 秩相关性分析结果显示，NSCLC 患者癌组织标本中 nm23阳性表达率与 EGFR 阳性表达率呈负相关（rs =-0.175，P ﹤0.05）。结论 NSCLC 患者癌组织中 nm23呈低表达，EGFR呈高表达，两者参与 NSCLC 的发生且 nm23与淋巴结转移有关。%Objective To analyze the expressions and clinical significance of nm23 and EGFR in patients with non -small cell lung cancer( NSCLC). Methods From February 2010 to February 2014 in Weifang Maternal and Child Health Hospital and the People's Hospital of Weifang,a total of 72 inpatients with NSCLC were selected as observation group,a total of 25 patients undergoing pulmonary bullae resection were selected as control group,carcinoma tissue of observation group and normal lung tissue of control group were collected,and immunohistochemical staining was used to detect the expressions of nm23 and EGFR. Results The positive expression rate of nm2 of carcinoma tissue was 66. 7% ,was statistically significantly lower than that of normal lung tissue of 84. 0% (P ﹤ 0. 05);the positive expression rate of EGFR of carcinoma tissue was 58. 3% , was statistically significantly higher than that of normal lung tissue of 16. 0% (P ﹤ 0. 01). No statistically significant differences of positive expression rate of nm2 or EGFR was found in NSCLC patients with different gender,different age,different pathological types,different differentiated degrees,different TNM stages,with smoking history or not(P ﹥ 0. 05);there was statistically significant difference of positive expression rate of nm2 in NSCLC patients with lymphatic metastasis or not( P ﹤0. 01),while there was no statistically significant differences of positive expression rate of EGFR in NSCLC patients with lymphatic metastasis or not(P ﹥ 0. 05). Spearman rank correlation analysis results showed that,the positive expression rate of nm2 of carcinoma tissue was negatively correlated with the positive expression rate of EGFR of carcinoma tissue in patients with NSCLC(rs = - 0. 175,P ﹤ 0. 05). Conclusion The expression of nm2 of carcinoma tissue is low in patients with NSCLC, while the expression of EGFR is high,nm23 and EGFR play important roles in the occurrence of NSCLC,and nm23 is correlated with the lymphatic metastasis.