非小细胞肺癌患者外周血Lunx mRNA的检测及意义

摘要

Objective To detect the expression of Lunx mRNA in peripheral blood of patients with non-small cell lung cancer( NSCLC ) and discuss the possibility of indicating lung cancer micrometastases by it. Methods Fluorescence quantitative reverse transcription- polymerase chain reaction ( FQ-RT-PCR )was used to detect Lunx mRNA in peripheral blood from 50 patients with NSCLC. 20 patients with benign pulmonary diseases and 30 healthy volunteers. Results In peripheral blood,the positive rate of Lunx mRNA for NSCLC was 56. 0% ( 28/50 )VS 0% ( 0/50 )in controls, The positive rate of Lunx mRNA in peripheral blood of NSCLC had a relationship with stage and had no relationship with age,gender,pathology type,and differentiation grade. Conclusion Lunx mRNA are efficient indicating factors on sensitivity and specificity to detect early haematogenous dissemination of cancer cells for patients with NSCLC. This method may lead to an earlier diagnosis of metastasis for lung cancer and do some help to evaluate the cancer more correctly and make the best treatment plan.%目的 探讨非小细胞肺癌患者外周血中人类肺组织特异性基因Lunx mRNA的表达情况,检验lunx mRNA作为肺癌微转移检测分子标志物的可行性.方法 以肺部良性疾病20例、健康人30例外周血为对照.采用实时荧光定量逆转录PCR(fluorescence quantitative reverse transcription- polymerase chain reaction,FQ-RT-PCR)法定量检测50例非小细胞肺癌患者外周血lunx mRNA的表达情况;并分析lunx mRNA的表达与各临床因素的关系.结果 50例非小细胞肺癌(non-small cell lung cancer,NSCLC)外周血lunx mRNA表达阳性者28例,阳性率为56.0%,而20例肺良性疾病和30例健康者外周血均不表达;同时发现lunx mRNA的表达与肺癌的分期明显相关,而与年龄、性别、病理分型、分化程度等无明显相关.结论 Lunx mRNA RT-PCR法检测非小细胞肺癌患者外周血微转移具有较高的特异性和敏感性,有助于早期诊断肺癌微转移,从而指导临床分期和治疗.