重组蛋白P53联合紫杉醇在宫颈癌中的抑制作用

摘要

Objective To detect the inhibitory effect of recombinant human P 53 combined with paclitaxel on human cer-vical cancer cell and its mechanism .Methods Human HeLa cell was used to detect the cell proliferation ,and add P53 or pacli-taxel alone ,and both of them as experiment ,blank as a control ,the congenic assay was detected ,and the protein was detected by western-blot.Results The growth of HeLa cells decreased after add P53 and paclitaxel,the P53 and paclitaxel alone also de-creased,P53 and paclitaxel had statistical difference ,P<0.001,and the ability of congenic decreased after add P 53 and paclitax-el,and the expression of VEGF decreased .Conclusion The inhibitory ability of recombinant human P 53 combined with paclitax-el on human cervical are efficient ,its potential mechanism of action is inhibition of the expression of VEGF .%目的 探讨P53联合紫杉醇在宫颈癌中的抑制作用.方法 利用宫颈癌细胞系HeLa细胞进行实验研究,利用MTT实验对单独加入P53重组蛋白、紫杉醇和P53联合紫杉醇对细胞增殖进行检测,两者均不加为对照组,同样的设计思路对细胞单克隆进行检测,最后用western-blot进行4种细胞蛋白的检测.结果 重组蛋白联合紫杉醇时抑制宫颈癌细胞的生长,且抑制作用具有统计学差异;细胞单克隆形成数目也是减少,在初步探讨其作用机制时发现VEGF表达减少.结论 P53重组蛋白联合紫杉醇能有效抑制宫颈癌细胞生长,其潜在作用机制是抑制VEGF的表达.