移植物抗肿瘤效应抗大肠癌的实验研究

摘要

Objective To build a comprehensive adjuvant therapy for colorectal cancer through graft versus tumor effect (GVT) and related cell factor.Methods Subcutaneous injection of CT26 tumor cells of BALB/C mice was conducted to establish the mouse model of colorectal cancer The mice model were randomized into different group and recorded survival times,tumor size and tumor growth inhibition rates.Results The group treated by DLI + CP survived (34.9 ± 2.75) days and the untreated group survived (15.4 ± 2.50) days (P ＜ 0.01).The tunor inhibitory rate was 94.3 ％ in DLI + CP group and CP or DLI alone group were 69.4％ and 74.4％.Conclusion GVT effect is helpful for the treatment of tumor,it is a new method for the treatment of solid tumor cells in the future.%目的 通过移植物抗肿瘤效应和相关的细胞因子共同作用,建立一套综合的大肠癌辅助治疗方案.方法 对BALB/C小鼠的皮下注射CT26肿瘤细胞来建立结肠癌小鼠模型,将CT26肿瘤模型鼠随机分组治疗,记录它们的存活天数、肿瘤大小、肿瘤生长抑制率.结果 免疫细胞移植联合环磷酰胺(CP)治疗组的存活天数为(34.9±2.75)天,未治疗组存活天数(15.4±2.50)天(P＜0.01).联合治疗组肿瘤抑制率为94.3％.单独的CP治疗组和免疫细胞移植组肿瘤抑制率分别为99.4％和74.4％.结论 免疫细胞移植存在(GVT)效应,有益于肿瘤的治疗,是潜在的细胞免疫治疗实体瘤的新方法.