雌激素及精氨酸对去卵巢大鼠血清一氧化氮水平的影响

摘要

Objective To investigate the effects of estrogen replacement and L-arginine (L-Arg) supplementation on serum levels of nitric oxide in ovariectomized rats. Methods Forty female Wistar rats, 3-month-old, were randomly divided into four goups, goup A: sham-ovariectomized rats; group B: ovariectomized rats; group C: ovariectomized rats receiving estrogen replacement therapy; group D: ovariectomized rats receiving estrogen replacement and L-Arg supplementation therapy. Four months later, the concentrations of serum NO, E2 and L-Arg were assayed by nitrate reductase method, RIA and HPLC, respectively. Results (1) The concentrations of serum NO and E2 in group B was decreased significantly compared with other groups (P <0. 05). (2) The serum levels of E2 had no significant difference in group A, C and D (P > 0. 05). (3) The serum levels of NO had no significant difference in group C and A (P >0. 05). (4) The serum levels of NO in group D was increased significantly compared with group C (P < 0. 05 ). Conclusions ( 1) Estrogen could increase the concentration of serum NO in ovariectomized rats. (2) Exogenous L-Argsupplement could increase the concentration of serum NO in ovariectomized rats.%目的 观察雌激素及精氨酸补充治疗对去卵巢大鼠血清一氧化氮(NO)水平的影响. 方法 3月龄雌性Wistar大鼠40只,随机分为4组,A组:假手术组；B组:去卵巢组；C组:雌激素治疗组；D组:雌激素+L-精氨酸(L-arginine,L-Arg)治疗组.4月后用硝酸还原酶法测NO3-/NO2-(的浓度来反映血清NO的浓度,并分别用放射免疫技术和高效液相色谱法检测血清中的雌二醇(E2)和L-Arg含量. 结果 (1)和A组、C组、D组相比,B组E2和NO浓度显著降低,差异有统计学意义(P＜0.05).(2)C组和D组、A组相比E2浓度差异无统计学意义(P＞0.05).(3)C组和A组相比NO浓度无统计学差异(P＞0.05).(4)D组和C组相比NO浓度升高有统计学差异(P＜0.05). 结论 E2可增加去卵巢大鼠血清NO水平.外源性L-Arg补充可提高血清NO水平.