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杀虫剂对橘小实蝇毒力测定方法的筛选和应用

         

摘要

Four methods, including residual film, topical application, paper scroll with insecticide film and feeding, were applied to test the toxicity of trichlorfon, beta-cypermethrin and abamectin to the adults of the oriental fruit fly B. Dorsalis (Hendel) in this study. The results showed that not all bioassay methods were suitable for testing the toxicity of different insecticides. The four methods above were applicable for toxicity test of trichlorfon to B. Dorsalis adults, while feeding and paper scroll with insecticide film method not for beta-cypermethrin and abamectin, respectively. Topical application and residual film methods were applied to monitor the resistance of Guangzhou population of B. Dorsalis to phoxim, trichlorfon, cyhalothrin, beta-cypermethrin, cypermethrin, emamectin benzoate and abamectin. The results indicated that the resistance to pyrethroids with residual film method was slightly lower than that with topical application method. The results with the topical application and residual film methods could well represent insecticide resistance in field populations.%应用药膜法、点滴法、圆筒滤纸法和饲喂法测定了敌百虫、高效氯氰菊酯和阿维菌素对橘小实蝇成虫的毒力.结果表明,不同的药剂所适用的毒力测定方法并不完全相同.4种方法均适用于敌百虫对橘小实蝇成虫的毒力测定,饲喂法不适用于高效氯氰菊酯,圆筒滤纸法不适用于阿维菌素.应用点滴法和药膜法测定了橘小实蝇广州种群对辛硫磷、敌百虫、氯氟氰菊酯、高效氯氰菊酯、氯氰菊酯、甲维盐、阿维菌素的抗性程度.药膜法所测得的橘小实绳对拟除虫菊酯类农药的抗性倍数略低于点滴法.点滴法和药膜法的测定结果均能一致反映橘小实蝇田间种群对杀虫剂的抗性水平.

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