北京平谷区苹果锈果类病毒检测初报

摘要

采集北京市平谷区‘中秋王’、‘寒富’苹果叶片样品共12份，以 Northern 杂交、RT-PCR 方法检测苹果锈果类病毒（Apple scar skin viroid ，ASSVd）带毒情况。结果表明，表现明显锈果症状的‘中秋王’样品均检出 ASSVd，无明显症状的‘中秋王’样品没有检出 ASSVd，而无明显症状的‘寒富’样品全部携带 ASSVd。测序发现，所得序列与 NCBI 中已报道的 ASSVd 基因组序列相似性为91％～99％，且两品种中 ASSVd 主流序列完全相同。结果说明‘中秋王’对 ASSVd 较为敏感，感病后易于表现出明显的锈果症状；‘寒富’对 ASSVd 耐性较强，感病后不表现出明显症状。%Twelve ‘Zhongqiuwang’and ‘Hanfu’apple leaf samples were collected from Pinggu District,Beijing, China,and Apple scar skin viroid (ASSVd)was detected in these samples using Northern blot and RT-PCR.The results showed that symptomatic ‘Zhongqiuwang’and asymptomatic ‘Hanfu’samples were infected by ASSVd, while no ASSVd was detected in asymptomatic ‘Zhongqiuwang’samples.Sequencing analysis showed that the ob-tained ASSVd sequences had 91%-99% similarities with other ASSVd sequences in the NCBI,and the dominant sequences of ASSVd from ‘Zhongqiuwang’and ‘Hanfu’were the same.Our results demonstrated that ‘Zhongqi-uwang’seems to be susceptible and sensitive to ASSVd,and ‘Hanfu’showed a relatively strong tolerance to ASSVd.