烟粉虱抗、感杀虫剂品系内生菌群落的差异分析

摘要

本研究对B型和Q型烟粉虱的吡虫啉或吡丙醚的抗、感品系进行16S rRNA细菌多样性测序分析,以明确内共生菌的种类和数量与烟粉虱生物型和抗性的关系.结果表明Candidatus Nitrososphaera、Bacillus、Sphingobium只在Q型烟粉虱中存在,而Candidatus Hamiltonella、Lactobacillus、Helicobacter等只在B型烟粉虱中存在,说明这些内共生菌可能与烟粉虱B、Q两种生物型的分化有关;内共生菌Rickettsia、Candidatus Portiera、Candidatus Hamiltonella、S24-7、Prevotella等在烟粉虱的新烟碱类杀虫剂吡虫啉抗性品系中明显多于敏感品系,其可能与烟粉虱对吡虫啉的抗性有关;在对保幼激素类似物杀虫剂吡丙醚的抗性品系中,Candidatus Hamiltonella-OTU7、Candidatus Portiera-OTU5、Candidatus Portiera-OTU190、Rickettsia-OTU4、Rickettsia-OTU2的数量明显多于敏感品系,说明这些内共生菌可能与烟粉虱对该类药物的抗性有关.%To study the relationships between biotypes and resistance of Bemisia tabaci and its endosymbionts,we compared the microbiota among different strains of B and Q biotypes that are susceptible or resistant to neonicotinoids orpypriproxyfen by using 16S rRNA amplicon sequencing technology.Candidatus Nitrososphaera,Bacillus and Sphingobsium were found in Q biotype strain only,whereas Candidatus Hamiltonella,Lactobacillus and Helicobacter were detected only in the B biotype strains.This suggested that these endosymbionts might be related to the differentiation of B/Q biotypes.Rickettsia,Candidatus Portiera,Candidatus Hamiltonella,S24-7 and Prevotella were significantly enriched in the imidacloprid resistant strains than in the susceptible strain,suggesting that these endosymbionts might contribute to B.tabaci's resistance to imidacloprid.Furthermore,the pyriproxyfen-resistant strain carried significantly more Candidatus Hamiltonella-OTU7,Candidatus Portiera-OTUS,Candidatus Portiera-OTU190,Rickettsia-OTU4 and Rickettsia-OTU2 than the susceptible strain,implicating that these endosymbionts might be involved in B.tabaci's resistance to pyriproxyfen.Taken together,the data obtained support the involvements of endosymbionts in B/Q differentiation and their resistance to insecticides.