将无根和囊的紫萍(Spirodela polyrrhiza)P143品系半叶状体分别放在含有和不含6-苄基嘌呤的培养液上于长日照条件下培养.在不含6-BA的培养液上培养4 d以后,半叶状体开始失绿,光合能力降低;第12天时,半叶状体已接近死亡.但是,培养在含有6-BA的培养液上的半叶状体干重、叶绿素和可溶性蛋白质含量、希尔反应活力、核酮糖-1,5-二磷酸羧化酶/加氧酶的小亚基和其编码基因rbcS mRNA水平显著增加.这些6-BA处理过的半叶状体平均寿命可达 80 d,几乎等同于完整植物体上叶状体的寿命,因此提出细胞分裂素对于紫萍叶状体生物学功能的维持是必需的.%Spirodela polyrrhiza P143 half-fronds without roots and pockets were cultivated on liquid medium under long-day conditions with or without 6-benzyladenine(6-BA). After 4 d of cultivation on a medium without 6-BA the half-fronds began to lose their chlorophyll and decrease in photosynthetic activity, and on the 12th d of cultivation these half-fronds were almost dead. But when cultivated on the medium to which 6-BA was added half-fronds increased significantly their dry weight, chlorophyll and soluble protein contents, capacity of Hill reaction and the levels of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit and the rbcS mRNA encoding it. The 6-BA-treated half-fronds had a mean longevity of 80 d, almost the same as that of the intact fronds. Therefore, it is proposed that cytokinins are necessary for maintaining the activities of duckweed fronds.
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