二茂铁修饰碳糊电极示差脉冲伏安法测定槲皮素

摘要

制备了二茂铁修饰碳糊电极,并采用循环伏安法研究了槲皮素在该修饰电极上的电化学行为.研究发现:在pH6.2磷酸盐缓冲溶液中,修饰电极对槲皮素有良好的电催化作用,得到了一对氧化还原峰,其氧化反应是受吸附控制的两电子、两质子电极过程.并在此基础上提出了一种测定槲皮素的示差脉冲伏安法.试验结果表明:槲皮素的氧化峰电流与其浓度在8×10-4～2×10-6mol·L-1范围内呈线性关系,检出限(3S/N)为7×10-6mol· L-1.%Ferrocene modified carbon paste electrode was prepared, and the electrochemical behavior of quercetin of this modified electrode was studied by cyclic voltammetry. It was found that significant electro-catalytic action of the modified electrode was observed on the reaction of quercetin at the electrode, giving a pair of oxidation and reduction peak in PBS of pH 6. 2. The oxidation of quercetin at the modified electrode is controlled mainly by adsorption in an electrode process involved with 2 electrons and 2 protons. Based on this fact, a method of differential pulse voltammetry was proposed for the determination of quercetin. Linear relationship between values of oxidation peak current and concentration of quercetin was obtained in the range from 8×10-4 to 2 ×10-6 mol· L-1, with detection limit (3S/N) of 7×10-6 mol ·L-1.