溶液捕集-高效液相色谱法测定卷烟主流烟气中主要羰基化合物

摘要

Carbonyl compounds in mainstream of cigarette smoke were trapped in a mixture solution of acetonitrile and H3 PO4 ( containing 2, 4-dinitrophenylhydrazine), and derivatized simultaneously thereia The derivatized products obtained were taken for HPLC analysis. Merck Lichrosphere RP C18 column was used as separation column, and mixtures of component A, B and C mixed in various ratios were used in the gradient elutioa Component A and B are mixtures of water, acetonitrile, tetrahydrofuran and iso-propand with 2 different ratios of (59+30+10 + 1) and (33 + 65 + 1 + 1) respectively, and component C is acetonitrile. UV-detection with the wavelength of 365 nm was adopted in the determination. Values of detection limit (3S/N) of the method were found in the range of 0. 04 —0. 12 μg · g-1. The proposed method was applied to the analysis of mainstream of cigarette smoke, giving values of recovery in the range of 75. 0% — 96. 0% and RSD's (n=5) in the range of 2. 0%—9. 3%.%卷烟主流烟气中的羰基化合物用含2,4-二硝基苯肼的乙腈-磷酸溶液为吸收液进行捕集,并在此溶液中使羰基化合物衍生化,所得衍生化产物供高效液相色谱分析.采用Merck Li-chrosphereRP C18色谱柱进行分离,并用不同配比混合的水-乙腈-四氢呋喃-异丙醇(59+30+10+1)、水-乙腈-四氢呋喃-异丙醇(33+65+1+1)和乙腈的混合液作为流动相进行梯度淋洗,用紫外检测器于波长365 nm处测定.方法的检出限(3S/N)在0.04～0.12μg·g-1之间.方法用于卷烟主流烟气的分析,回收率在75.0％～96.0％之间,测定值的相对标准偏差(n=5)在2.0％～9.3％之间.