鸡屎藤次苷在大鼠体内的药动学研究

摘要

Objective: To develop an HPLC-UV method for the determination of scandoside in rat plasma, and to study pharmacokinetics of scandoside in rats in vivo. Methods: HPLC -UV method was used to determine the scandoside in rat plasma. The separation was performed on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with methanol-aeetonitrile-water (3:2:95, v/v/v, containing 0.1% acetic acid) as the mobile phase at the flow rate of 1.0 mL·min-1. The UV detection was set at 230 nm and the column temperature was 25℃. Results: The calibration curves were linear over the concentration range 0.2～40 μg· mL-1 (Y=0.07321X-0.00939, r=0.9940) in rat plasma was 0.2 μLg ·mL -1. The intra-day and inter-day precisions were within 7.1% and 13.5%, respectively, and RE was -4.5%～1.8%. The average extraction recoveries were 64.5%～73.1%. Following intravenous administration in rats (4.2 mg·kg-1), the primary phamacokinetic parameters of scandoside in plasma were as follows: t112 (33.5±4.39) min, AUC0-180 min (922± 129) (μg·min ·ml-1. Conclusion: This method is selective, accurate and sensitive, which can be applied for the determination of scandoside in plasma and its pharmacokinetic studies.%目的:测定大鼠血浆中鸡屎藤次苷的含量,并研究其在大鼠体内的药动学过程.方法:采用HPLC -UV法测定大鼠尾静脉注射给药后血浆中鸡屎藤次苷的血药浓度.色谱条件为:DiamonsilTM C18柱(250 mm×4.6 mm,5μm),流动相:甲醇一乙腈-0.1％冰醋酸(3:2:95,v/v/v),流速:1.0 mL·min-1,检测波长:230 nm.通过BAPP2.0软件求算其药动学参数.结果:鸡屎藤次苷血浆浓度在0.2～40μg·mL-1(Y=0.07321X-0.00939,γ=0.9940)范围内线性关系良好.日内精密度不大于7.1％,日间精密度不大于13.5％,准确度RE值在一4.5％～1.8％之间.鸡屎藤次苷提取回收率为64.5％～73.1％,内标物提取回收率为74.5％.主要药动学参数为t1/2=(33.5+4.39) min,AUC0-180mm=(922±129) μg·min· mL-1.结论:该法专属、准确、灵敏,适用于鸡屎藤次苷在大鼠体内的药动学研究,为进一步研究鸡屎藤次苷体内药动学行为提供了依据.