HPLC-ELSD 法测定不同产地小茴香中亚油酸含量

摘要

目的：建立小茴香中亚油酸含量的 HPLC-ELSD 测定方法并测定不同产地小茴香的亚油酸含量。方法：采用 Cosmosil 5 C18-MS-Ⅱ柱，甲醇-水-冰醋酸（95∶5∶0.1%），流速1.0 mL· min-1，柱温35℃，漂移管温度40℃，雾化气 N2，载气流速3.0 L·min-1。结果与结论：本法测定小茴香中亚油酸含量简便可靠。测得4个产地小茴香中亚油酸含量最高为8.73 mg·g-1，最低为1.70 mg·g-1。不同产地小茴香中亚油酸含量差异明显。%Objective: To establish a quantitative HPLC-ELSD method for the determination of linoleic acid in Foeniculum vulgare Mill. from different habitats. Methods: A column of Cosmosil 5C18-MS-Ⅱ was used with the mobile phase of methanol and water and acetic acid (95∶5∶0.1%), the fIow rate was 1.0 mL· min-1, and the column temperature was set at 35 ℃. The temperature of drift tube was set at 40 ℃ with an air fIow rate of 3.0 L·min-1 for ELSD. Results and Conclusion: The established HPLC-ELSD method is simple and suitable for the quality control of Foeniculum vulgare Mill. The maximum content of linoleic acid in Foeniculum vulgare Mill. from four habitats was 8.73 mg·g-1 and the minimum content was 1.70 mg· g-1. Great difference of linoleic acid content is shown in Foeniculum vulgare Mill. from different habitats.