20％溴硝醇可湿性粉剂高效液相色谱分析方法研究

摘要

Bronopol 20％ WP has obtained full registration on rice to control bakanae disease in China.A method for the separation and quantitative analysis of bronopol by HPLC with methanol and 0.04％ phosphoric acid-acetonitrile (20:80 v/v) as mobile phase,SinoChrom ODS-BP-C1scolumn and DAD at 215 nm wavelength was described.The results showed that the method is valid over a range of 0.224 8 ～ 1.124mg/mL with the correlation coefficients of above 0.998 8,the standard deviation of 0.11 and the variation coefficient of 0.55％.The average recovery over this range was 99.8％.%20％溴硝醇可湿性粉剂在水稻上已获得正式登记,用以防治水稻恶苗病.本文采用高效液相色谱法,以甲醇:0.04％磷酸水溶液(20:80)为流动相,使用SinoChrom ODS-BP-C18、5μm为填料的不锈钢柱和二极管阵列检测器,在215nm波长下对溴硝醇可湿性粉剂进行分离和定量分析.结果表明,该分析方法在0.224 8～1.124mg/mL线性范围内,相关系数为0.998 8,标准偏差为0.11,变异系数为0.55％,平均回收率为99.8％.