辛伐他汀促进种植体骨整合的实验研究

摘要

目的：在建立大鼠上颌骨牙种植模型的基础上，探讨口服辛伐他汀对种植体周围骨整合的影响。方法：3月龄雌性SD大鼠在上颌骨第一磨牙前方植入0．8 mm×2．0 mm纯钛种植体，随机分为对照组和实验组，每日分别给予生理盐水和辛伐他汀灌胃。术后1、2、4周检测大鼠体质量、血清碱性磷酸酶（ALP）和骨钙素（BGP）表达量，HE染色、microCT检测观察术后种植区新骨形成情况，并对各组之间的成骨差异进行统计分析。结果：辛伐他汀实验组的大鼠体质量要轻于对照组，差异有统计学意义（P＜0．05）；实验组血清ALP和BGP明显高于对照组，且差异有统计学意义（P＜0．05）。 HE染色表明，2周时实验组新骨明显增加，对照组有少量新骨形成；microCT显示，4周时两组间骨小梁厚度、骨小梁数目、骨体积百分比：实验组＞对照组，骨小梁间距：实验组＜对照组，且差异都有统计意义（ P＜0．05）。结论：口服辛伐他汀对种植体周围新骨的形成有明显的促进作用。%Objective:To investigate the effect of simvastatin administration on osseointegration surrounding the implant by estab⁃lishment of maxillary implant rat model. Methods:Three⁃month⁃old female SD rats were randomly divided into control group and oral administration group. The control group was administrated with saline. The other group was administrated with simvastatin. After Titani⁃um implants(0.8 mm×2.0 mm)were put in anterior to the first molar of maxillary, different administration programs were applied and the rats weights and serum concentrations of ALP and BGP were detected at 1, 2 and 4 weeks, respectively. Two weeks after adminis⁃tration, HE staining was used to observe the formation of new bone. After 4 weeks administration, micro⁃CT was used to observe the bone structure by measuring bone density around the implant. Results:The weight of rats was decreased in simvastatin administration group compared with that of the control group, and the difference was statistically significant ( P< 0.05) . The data of serum ALP and BGP showed the similar trend with weight (P< 0.05). At 2nd week, HE staining indicated that much more trabecular bone was ob⁃served in simvastatin administration rats in contrast with control. At 4th week, the data of all the trabecular bone parameters were in⁃creased in simvastatin administration rats, except for Tb.Sp and the difference was statistically significant (P< 0.05). Meanwhile, three⁃dimensional reconstruction also confirmed the similar results. Conclusions: Simvastatin significantly promotes the formation of new bone around the implant via orally administration.