香菇多糖对口腔鳞癌细胞增殖的影响

摘要

Objective: To investigate the effect of lentinan on the proliferation of oral squamous cell carcinoma cell HN4 and its mechanism.Methods: In vitro MTT assay was used to detect the growth inhibition of HN4 cells.The effect of lentinan on the apoptosis of HN4 cells was detected by AO/EB method.The expression of Bcl-2 and Bax in HN4 tumor cells was detected by Western blot.Re-sults: Lentinan could significantly inhibit the growth and proliferation of oral squamous cell carcinoma HN4 cells.AO/EB staining showed that after lentinan stimulated the cells,apoptosis of HN4 cells was observed under a fluorescence microscope,staining in-creased,nuclear pyknosis and orange-red staining were observed.Western blot results showed that lentinan could decrease the expres-sion of Bcl-2,increase the expression of Bax and increase the ratio of Bax/Bcl-2 in HN4 cells.Conclusions: Lentinan could inhibit the proliferation of oral squamous cell carcinoma HN4 cells in vitro and induce the apoptosis of HN4 cells.%目的:探讨香菇多糖对口腔鳞癌细胞HN4增殖的影响.方法:体外MTT法检测香菇多糖对HN4细胞的生长抑制作用;AO/EB法研究香菇多糖诱导HN4细胞凋亡的作用;Western blot检测HN4肿瘤细胞中Bcl-2、Bax的表达情况.结果:香菇多糖体外能显著抑制HN4细胞的生长增殖.AO/EB染色表明香菇多糖刺激细胞后,荧光显微镜下能观察到HN4细胞凋亡,出现染色增强,核固缩,染成橙红色.Western blot结果显示香菇多糖能降低HN4细胞中Bcl-2的表达,增加Bax的表达,并增加Bax/Bcl-2的比值.结论:香菇多糖能够体外抑制HN4细胞的生长增殖,并且能够诱导HN4细胞凋亡.