Objective To investigate the expression and clinical significance of serum thymidine kinase 1 (S-TK1). Method 223 patients with laryngeal squamous cell carcinoma were included as study group, of which the fasting blood samples before surgery, 1 week after surgery, and 1 month after surgery were collected, respectively, and another 280 cases of normal subjects were enrolled as control group, with blood samples collected. All blood samples were determined for S-TK1 concentration, and the positive rates were compared between the two groups. Result The concentration of S-TK1 in study group befor surgery was (3.06±0.16) pmol/L, higher than the (1.28±0.75) pmol/L in control group, the difference was statistically significant (P<0.05); the positive rate of S-TK1 in study group before surgery was 50.22％ (112/223), which was significantly higher compared with control group at 13.57％(38/280), with significant difference observed (P<0.01). As for the S-TK1 concentration and positive rates among patients with stage Ⅰ, Ⅱ, Ⅲ and Ⅳ disease, similar results were observed (P>0.05);same findings were noted for subgroups of Ⅰ+Ⅱ stage vs Ⅲ+Ⅳ stage (P>0.05). Patients with moderately or poorly differentiated disease had higher positive rate of S-TK1 as compared with those with highly differen-tiated disease, and significant difference was observed (P<0.05);while patients with highly and moderately differentiated disease were lower in S-TK1 concentration as compared with those with poorly differentiated disease (P<0.05). The two measures before and 1 week after surgery in laryngeal squamous cell carcinoma showed no significant variations (P>0.05);while 1 month after surgery, both the concentration and positive rate of S-TK1 were decreased compared with be-fore surgery, and the difference was of statistically significance (P<0.05). Conclusion The concentration of S-TK1 can be used as one of the indicators for diagnosis and postoperative evaluation of laryngeal carcinoma, of which the expres-sion level is associated with the histological grading of laryngeal squamous cell carcinoma, and the concentration may help determine the malignant degree.%目的 探讨喉鳞状细胞癌患者血清细胞质胸苷激酶1(S-TK1)的表达水平及临床意义.方法 选取223例喉鳞状细胞癌患者为试验组,采集患者术前、术后1周、术后1个月空腹血液样本各1份;另选取280例健康体检者为对照组,采集血液样本.检测所有血液样本的S-TK1浓度,比较两组研究对象的S-TK1浓度及阳性率.结果 术前试验组患者的S-TK1浓度为(3.06±0.16)pmol/L,高于对照组的(1.28±0.75)pmol/L,差异有统计学意义(P﹤0.05);术前试验组患者的S-TK1阳性率为50.22％(112/223),明显高于对照组的13.57％(38/280),差异有统计学意义(P﹤0.01).Ⅰ、Ⅱ、Ⅲ、Ⅳ期喉鳞状细胞癌患者的S-TK1浓度及阳性率比较,差异均无统计学意义(P﹥0.05);Ⅰ+Ⅱ期与Ⅲ+Ⅳ期喉鳞状细胞癌患者的S-TK1浓度及阳性率比较,差异均无统计学意义(P﹥0.05).中分化、低分化喉鳞状细胞癌患者的S-TK1阳性率均高于高分化患者,差异有统计学意义(P﹤0.05);高分化、中分化喉鳞状细胞癌患者的S-TK1浓度均低于低分化患者,差异有统计学意义(P﹤0.05).术前喉鳞状细胞癌患者的S-TK1阳性率和S-TK1浓度与术后1周比较,差异均无统计学意义(P﹥0.05);术后1个月喉鳞状细胞癌患者的S-TK1阳性率和浓度均低于术前,差异有统计学意义(P﹤0.05).结论 S-TK1浓度可以作为喉鳞状细胞癌诊断与术后评估的指标之一,其表达水平与喉鳞状细胞癌的组织学分级有关,可通过其浓度分析喉鳞状细胞癌的恶性程度.
展开▼
