外源性神经生长因子治疗大鼠脑出血的疗效分析

摘要

Objective:To observe the therapeutic effect of exogenous nerve growth factor (NGF) on rats after intracerebral hemorrhage(ICH). Methods:Forty-eight SD rats were randomly divided into NGF group (n=24), ICH group (n=12) and sham group (n=12). ICH models were made by injecting Ⅶ collagenase into caudate nucleus of rats. Rats in NGF group were treated with NGF whereas those in ICH group received normal saline. The neurolog-ical function was evaluated, and the Nestin and vascular endothelial growth factor (VEGF) positive cells located perihematoma were detected by immunohistochemistry at 1, 3 and 7 days after operation respectively. Results:The neurological severity score at 7 d after operation was lower than those at 1 and 3 days after operation in NGF group ( <0.05). The neurological severity score of NGF group was lower than that of ICH group at 7 days after operation ( <0.05). The numbers of VEGF and Nestin positive cells in NGF group were significantly higher than those in sham group ( <0.01) and those in ICH group ( <0.05) at 1, 3, 7 days after operation. The numbers of VEGF and Nestin positive cells at 7 days after operation was greater than those at 1 and 3 days after operation in NGF group ( <0.05). Conclusion:Exogenous NGF contributes to the improvement of neural function recovery of rats after ICH, which may be mediated by activation of endogenous neural stem cells and increase of VEGF expression.%目的：观察外源性神经生长因子（NGF）治疗大鼠脑出血的疗效。方法：48只SD大鼠随机分成NGF组24只、脑出血组12只和假手术组12只。Ⅶ型胶原酶尾状核注射制备大鼠脑出血模型；NGF组给予NGF血肿内注射，脑出血组注射等量生理盐水，假手术组只进针。分别于术后1、3及7d对3组大鼠进行神经功能评分，免疫组化法检测血肿灶周Nestin及血管内皮生长因子（VEGF）的阳性细胞数。结果：造模后7 d， NGF组神经功能缺损评分降低，低于NGF组1 d及3 d时的评分（＜0.05），且低于脑出血组7 d时的评分（＜0.05）。各时间点NGF组Nestin及VEGF阳性细胞数均显著高于假手术组（＜0.01），高于脑出血组（＜0.05）；NGF组7 d时Nestin及VEGF阳性细胞数高于同组1 d及3 d时（＜0.05）。结论：外源性NGF有助于促进脑出血大鼠的神经功能恢复，其机制可能与激活脑出血大鼠的内源性神经干细胞及促进脑组织分泌VEGF有关。