HPLC测定葛花中6″-O-木糖鸢尾苷的含量

摘要

An HPLC method for determination of 6"-0-xylosyl-tectoridin in Flos Puerariae was developed. The samples were separated on a C18(250 mm x 4.6 mm,5 μm) column with the mobile phase of acetonitrile-water (gradient elu-tion ). Plow rate was 0.8 mL/min. The detection wavelength was set at 265 ran. Column temperature was ambient temperature. The linear range of 6"-0-xylosyl-tectoridin was 10.33-185.99 (μg/mL.and the linear function was Y= 3073IX -216635 (r = 0.9992). The average recovery was 100.2% ,and RSD was less than 2% ( n =9). The contents of 6"-0-xylosyl-tectoridin in Flos Puerariae was 11.08-48.23 mg/g. The method was accurate and reliable,which can be used to evaluate the qualities of Flos Puerariae.%本文建立了葛花中6"-O-木糖鸢尾苷含量测定的HPLC方法.采用GRACE C18(250 mm× 4.6 mm,5μm)色谱柱,流动相为乙腈-水梯度洗脱,流速为0.8 mL/min,紫外检测波长为265nm,柱温为室温.6”-O-木糖鸢尾苷的峰面积(Y)与浓度(X)在10.33～185.99 μg/mL范围内线性关系良好,Y=30731X - 216635,r=0.9992(n =7)；平均回收率为100.2％,RSD＜2％(n=9)；测得8批葛花药材中6”-O-木糖鸢尾苷含量为11.08～ 48.23 mg/g.方法学验证结果表明该法准确、可靠,可用于葛花中主要成分6"-O-木糖鸢尾苷的含量测定.