大孔吸附树脂对金银花叶茎藤总黄酮的吸附性能

摘要

从金银花叶茎藤中提取总黄酮并用D-101大孔吸附树脂进行纯化,研究了D-101大孔吸附树脂对总黄酮的吸附及解吸附特性.结果表明,D-101树脂对金银花叶茎藤总黄酮分离纯化的最佳工艺参数为:上样液黄酮浓度0.538 mg/mL,静置吸附时间80 min,料液比1∶5(g∶mL),pH 2,流速为2 mL/min,以60 mL 75％的乙醇溶液洗脱,黄酮解吸率为94.5％,纯化后黄酮纯度为84.5％,是粗提液黄酮含量(16.8％)的5倍.金银花叶茎藤总黄酮在D-101树脂上的吸附等温线符合Langmuir等温吸附方程.吸附热力学参数表明吸附过程为自发、放热过程,吸附动力学可用Pseudo-second-order模型较好地拟合,30℃时其表观吸附速率常数为1.034×10-2g/mg·min.%The flavonoids were extracted from leaves and rattan of Lonicera japonicab and separated using D-101 macro-porous resin. The adsorption and desorption characteristics of flavonoids on macroporous resin were investigated. The results showed that under the conditions of 0.538 mg/mL of flavonoids extracts,80 min standing time,1:5 (g: mL) material-to-liquid ratio,pH 2,2 mL/min of flow rate and eluting with 60 mL of 75% ethanol.the desorption rate was 94.5%. The percentage of flavonoids in the purified sample reached 84.5% ,5 times of that (16.8% ) of the crude extract. The adsorption isotherms of the flavonoids on the D-101 resin were consistent with Langmuir isotherm equations. The dynamic parameter results showed that the absorption process was a spontaneous endothermic procedure. The adsorption was described well by the Pseudo-second-order kinetics. The apparent adsorption rate constants was 1.034 × 10-2g/mg · min.