本文建立了金银花中芦丁、金丝桃苷和槲皮素的HF-LPME-UHPLC-MS检测方法.应用中空纤维液相微萃取技术作为前处理方法:聚丙烯中空纤维为支架,乙酸乙酯为有机溶剂,pH 3.0的KH2PO4溶液为给出相,pH 8.5的NaHCO3溶液为接收相,搅拌速度为1000 rpm,萃取时间为50 min.用UHPLC-MS作为检测手段,检测结果为:芦丁、金丝桃苷和槲皮素的线性范围分别为0.0227～1.195、0.0141 ～ 1.32、0.00832～1.649 μg/mL,平均回收率为别为95.34％、95.17％、99.83％,富集倍数为别为38、42、83,检测限分别为0.029、0.017、0.006μg/L.方法学验证结果表明该法灵敏、准确、提高了检测限和富集倍数,可用于检测金银花中的黄酮物质.%A hollow fiber-based liquid-phase microextraction combined with ultrahigh performance Liquid chromatogra-phy-mass spectrometry (HF-LPME-UHPLC-MS) method was developed,optimized and validated for the simultaneous determination of rutin, hyperin and quercetin in Lonicera japonica. HF-LPME was used for sample preparation; use polypropylene hollow fiber as holder, ethyl acetate as sol vent, KH2PO4(pH 3.0) as donor phase, NaHCO3 ( pH 8.5) as acceptor phase, 1000 rpm as stirring rate and 50 min as extraction time. UHPLC-MS was used for sample testing. The results were that:the linear range of rutin,hyperin and quercetin were 0.0227-1. 195,0. 0141-1. 32 and 0. 00832-1.649 μg/mL;the average recoveries of rutin,hyperin and quercetin were 95.34% ,95.17% and 99.83% ;the enrichment factors of rutin, hyperin and quercetin were 38,42 and 83; the detection limits of rutin, hyperin and quercetin were 0.029, 0.017 and 0.006 μg/L. This method had been proven to be accurate and reliable. It can be used to evaluate the qualities of L japonica.
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