深海来源链霉菌Streptomyces griseorubens SCSIO ZY0206多酚蒽酮类代谢产物的研究

摘要

从中国南海北部3563 m深的沉积物中分离获得放线菌SCSIO ZY0206,经16S分子生物学鉴定为Streptomyces griseorbens.其发酵产物具有抗菌活性,进而以抗菌活性为指导,采用硅胶柱层析及半制备高效液相色谱法对其代谢产物进行追踪分离,得到4个多酚蒽酮类化合物,经HR-ESI-MS、ESI-MS、1H及13C NMR和HMBC谱鉴定为resistoflavine(1)、resistomycin(2)、1-hydroxy-l-norresistomycin (3)和tetracenomycin D (4).%An actinomycete strain,SCSIO ZY0206,was isolated from a marine sediment collected at a depth of 3563 m in the northern South China Sea. This deep sea-derived actinomycete was identified as Streptomyces griseorubens by analysis of its 16S rRNA gene sequence. The fermentation broth of SCSIO ZY0206 showed antibacterial activity. Chemical investigation of the culture of this strain was carried out using silica gel column chromatography and semi-preparative HPLC. Four known compounds,resistoflavine (1) ,resistomycin (2) , 1 -hydroxy-1 -norresistomycin (3) and tetracenomycin D (4) were obtained. The structures of these compounds were identified on the basis of extensive spectroscopic methods, including HR-ESI-MS,1H and 13C NMR,and HMBC.