海洋真菌Penicillium oxalicum SCSGAF 0023的次生代谢产物及其抗污损和酶抑制活性研究

摘要

Sixteen compounds were isolated from marine-derived fungus Penicillium oxalicum SCSGAF 0023 strain. They were identified to be n-butyl isobutyl terephthalate (1) , dibutyl terephthalate (2) , 1, 3,7-trihydroxy-6-methylanthra-quinone(3), 1,6,7-trihydroxy-3-methoxy-anthraquinone (4), isorhodoptilometrin (5), citreorosein (6), emodin (7) , methyl-3,8-dihydroxy-6-methyl-9- oxo-9H-xanthene-1-carboxylate (8) , pinselin (9) , secalonic acid D (10) , quinolactacin C,(11) , quinolactacin C2(12) , quinolactacin A1 (13) , quinolactacin A2 (14) , quinolactacin B1 (15) , and 3-methyl-lH-indole-2-carboxylic acid (16) on the basis of spectroscopic analysis and comparison with literatures. Compound 1 was a new natural product. Compounds 5 and 7 showed strong antifouling activity with EC50 values of 3. 8 and 6. 0 μg/ml, respectively. Compound 10 exhibited moderate inhibitory activity against PTPIB with IC50 value of 24.0 μM, and 15 showed moderate inhibitory activity against cathepsin B with IC50 value of 16.0 μM.%利用pTLC、硅胶、Sephadex LH-20及半制备HPLC等柱色谱手段,从海洋真菌Penicillium oxalicum SCSGAF 0023的次生代谢产物中分离得到16个化合物,波谱学数据分析鉴定为n-butyl isobutyl terephthalate(1),dibutyl terephthalate(2),1,3,7-trihydroxy-6-methylanthraquinone(3),1,6,7-trihydroxy-3-methoxy-anthraquinone (4),isorhodoptilometrin (5),citreorosein (6),emodin (7),methyl-3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8),pinselin (9),secalonic acid D(10),quinolactacin C1(11),quinolactacin C2(12),quinolactacin A1 (13),quinolactacin A2 (14),quinolactacin B1(15),3-methyl-IH-indole-2-carboxylic acid (16).其中,化合物1为新天然产物,化合物5和7表现出强的抗草苔虫幼虫附着活性,EC50值分别为3.8和6.0 μ.g/mL;化合物10和15分别对PTPIB和cathepsin B显示中等的酶抑制活性,IC50值分别为24.0和16.0 μM.