The aim of this study was to optimize the green synthesis process of emblicpolysaccharide-silver nanoparticles.The Ag+ of AgNO3 was reduced to the silver level of nano-grade using emblic polysaccharide as reducing agent and stabilizer.On the basis of single factors,the inhibitory rate of AgNPs against Staphylococcus aureuswas the response value,the effects of AgNO3 and polysaccharide volume ratio,NaC1 volume and UV irradiation time on the preparation of AgNPs was studied.According to the principle of Box-Benhnken center combination test,three factors and three levels were used to determine the optimal process.The results showed:the AgNPs particles had significant bacteriostatic effect on S.aureus.The optimal preparation conditions of AgNPs were as follows:the ratio of AgNO3 to polysaccharide was 2∶1 (mL/mL),the volume of NaCl was 1.5 mL,and the UV irradiation time was 1 h.Under these conditions,the diameter of the inhibition zone was 2.79 ± 0.01 cm.%为了优化绿色合成的余甘多糖制备纳米银复合粒子的制备,以余甘多糖为还原剂和稳定剂,将AgNO3中的Ag+还原为纳米级别的银单质.以AgNPs对金黄色葡萄球菌的抑菌圈为响应值,研究AgNO3与多糖体积比、NaC1体积、紫外照射时间对AgNPs制备工艺的影响,根据Box-Benhnken中心组合实验的原理采用三因素三水平的分析方法确定最佳工艺.研究结果表明:绿色合成的AgNPs粒子对金黄色葡萄球菌有显著的抑菌效果,最终确定最优AgNPs制备条件为:AgNO3与多糖体积比2∶1(mL/mL)、NaC1体积为1.5 mL、紫外照射时间为1h,测得抑菌圈直径大小为2.79 ±0.01 cm.
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