Objective To study the possible effect of endogenous carbonmonoxide(CO) on apoptosis of PC12 cellin hypoxia culture. Methods PC12 cellwere divided into 3 groups, normoxia group, hypoxia and hypoxia+ZnPP group, and each groupwas subsequently divided into 3 sub-groups which was cultured 1h, 2h and 6h respectively. The expression of HO at 2h. The level of endogenous CO and rates of cell apoptosis at different time were observed respectively. Results The expression of HO-1 was significant but HO-2 didnt' express. CO level and cell apoptosis rates of H group were significantly higher than those of C and H+ZnPP groups at 1h, 2h and 6h(P<0.01). These parameters of H+ZnPP group decreased as compared with H group(P<0.01). Conclusion HO-1 was highly expressed and endogenous CO was parently produced, in PC12 cell under hypoxia status, and rates of cell apoptosis were correlated with increased of en-dogenous CO leve. l HO/CO system may result in cell apoptosis.% 目的研究PC12细胞在低氧状态下,内源性一氧化碳(CO)的生成及其导致细胞凋亡的作用.方法将PC12细胞分为常氧对照组(C组)、低氧组(H组)和低氧加血红素氧合酶(HO)抑制剂卟啉锌-9组(H+ZnPP组).检测2h时间点PC12细胞血红素氧合酶的表达及内源性CO水平和细胞凋亡率.结果氧应激状态下,HO-1明显表达,HO-2无表达；H组HO水平与C组、H+ZnPP组比较明显升高(P<0.01),H+ZnPP组的HO水平高于C组(P<0.01)；内源性CO水平以及细胞凋亡率与C组、H+ZnPP组比较均明显升高(P<0.01).结论低氧状态下,PC12细胞表面HO-1高表达,HO-2无明显表达,PC12细胞的凋亡率与内源性CO的水平密切相关,提示HO/内源性CO可能导致细胞凋亡的发生.
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