目的：构建APCmin/+；Mac-1-/-小鼠模型。方法将自发肠腺瘤(APCmin/+)模型同β2家族整合素Mac-1缺失的小鼠(Mac-1-/-)进行杂交，剪小鼠尾巴抽提DNA，然后用PCR法鉴定小鼠的基因型。结果将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后，成功构建了APCmin/+；Mac-1-/-小鼠模型。结论将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后成功建立了APCmin/+；Mac-1-/-小鼠模型，进一步扩大种群。%Objective To establish the APCmin/+;Mac-1-/- mice. Methods Female PSGL-1 KO mice were mated with male APCmin/+ mice and male progeny carrying the APCmin/+ transgene. Mice were genotyped by PCR. Results After female Mac-1 KO mice were mated with male APCmin/+ mice for three generations, APCmin/+;Mac-1-/- mice were established. Conclusion APCmin/+;Mac-1-/- mice were obtained and propagated through APCmin/+ mice being mated with MAC-1 KO mice.
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