香栓菌的平喘及抗氧化活性研究

摘要

Treatment effect for OVA-induced allergic asthma and antioxidant activities of Trametes suaveolens fruiting body extract were investigated. Mice injected with OVA were used for establishment of allergic asthma model. Different dosage of the fruit body water extract (TSWE) and petroleum ether extract (TSPEE) were administrated to the mice. The morphological changes of lung tissue were observed, and the inflammatory cells in bronchoalveolar lavage fluid (BALF) were examined. Inflammatory cytokines (IgE, TNF-α, IL-1β, IL-6, IL-4, IFN-γ) in serum and lung homogenates were determined for each experimental group. The antioxidant activities of different polarity extract of the fruiting bodies were evaluated by determining scavenging rates against DPPH and ABTS free radicals. The result showed that TSWE moderate dose (120mg/kg) displayed a decrease of Eos in blood and BALF and IgE content in serum, and could reduce IL-4 and increase IFN-γ/IL-4 in serum and lung homogenate. TSPEE high dose (24mg/kg) displayed a decrease of Eos in blood and IgE, TNF-α, IL-1, IL-6 content in serum, and reduced lung tissue inflammatory cell infiltration. The results indicated that petroleum ether extract, chloroform extract, ethyl acetate extract and water extract of Trametes suaveolens fruiting bodies showed antioxidant capacity to different extent, presenting relation between dosage and curative effect. The antioxidant effect of water extract was remarkable. This study provides reference for treatment of respiratory system diseases by administrating Trametes suaveolens fruiting body extract.%本文对香栓菌子实体提取物的平喘作用和抗氧化活性进行研究.平喘药理实验采用鸡卵清蛋白(OVA)致敏法建立小鼠过敏性哮喘模型,灌胃给药香栓菌水提物(高240mg/kg、中120mg/kg、低60mg/kg剂量)和石油醚提取物(高24mg/kg、中12mg/kg、低6mg/kg剂量).比较各组小鼠行为变化,检测IgE、TNF-α、IL-1β、IL-6、IL-4和IFN-γ 等指标,分类计数血液及支气管肺泡灌洗液(BALF)中的嗜酸性粒细胞数量,分析肺组织病理变化;抗氧化实验采用香栓菌水提取物、乙酸乙酯提取物、氯仿提取物、石油醚提取物比较清除DPPH和ABTS自由基能力.平喘试验结果表明,香栓菌水提物中剂量(120mg/kg)能降低血中嗜酸性粒细胞(Eos)及血清中IgE含量,减少BALF中Eos,降低血清及肺组织匀浆中IL-4含量,提高IFN-γ/IL-4,减轻模型的病理改变;石油醚提取物高剂量(24mg/kg)能降低血中Eos和血清中IgE、TNF-α、IL-1、IL-6含量,减少肺组织炎症细胞浸润;抗氧化结果显示,香栓菌各层提取物均有不同程度的抗氧化能力,并呈现一定的量效关系,同时水提取物的抗氧化效果最显著.本文为深入研究香栓菌子实体对呼吸系统疾病的治疗提供了重要试验依据.