淫羊藿黄酮对去势大鼠骨生物力学性能的影响

摘要

目的：探讨淫羊藿黄酮对去势大鼠骨生物力学性能的影响。方法：选用2月龄雌性SD大鼠60只，随机分为6组，每组10只。A组：空白对照组，常规方法饲养，进行假手术处理；B组：去势组，不给予淫羊藿黄酮干预；C组：去势垣钙剂，每日灌服高效钙（75 mg·kg-1）垣维生素D321 IU·kg-1；D组：去势垣低剂量，每日灌服淫羊藿黄酮（75 mg·kg-1）；E组：去势垣中剂量，每日灌服淫羊藿黄酮（150 mg·kg-1）；F组：去势垣高剂量，每日灌服淫羊藿黄酮（300 mg·kg-1）。实验第4月末处死大鼠，取出大鼠双侧胫骨、双侧股骨、双侧肱骨和全部腰椎。实验动物干预完毕后测定：淤第四腰椎体力学性能指标中弹性模量、最大载荷、最大应力、变形位能、结构刚度，于股骨结构力学性能指标中最大载荷、破坏载荷、变形位能、结构刚度，盂股骨材料力学性能指标中弹性模量、最大应力、最大应变、破坏应力、破坏应变。结果：与B组比较，A、D、E、F组第四腰椎体力学性能指标中弹性模量、最大载荷、最大应力、变形位能、结构刚度，股骨结构力学性能指标中最大载荷、破坏载荷、变形位能、结构刚度，股骨材料力学性能指标中弹性模量、最大应力、最大应变、破坏应力明显升高（P<0.05）；C组有升高趋势；A、C、D、E、F组之间比较差异没有统计学意义；D、E、F组随淫羊藿黄酮剂量的增加而增加，但其相互之间差异没有统计学意义；股骨材料力学性能指标中破坏应变A、C、D、E、F组之间相互比较差异没有统计学意义；表明去势造成了大鼠椎骨力学性能，股骨结构力学性能和股骨材料力学性能的降低，应用淫羊藿黄酮可改善第四腰椎体力学性能、股骨结构力学性能和股骨材料力学性能。结论：淫羊藿黄酮可有效防治去势大鼠骨生物力学性能的降低，使其维持在较高水平。%This study was aimed to explore pharmacological effects of epimedium pubescen flavonoid (EPF) on biomechanical properties among ovariectomized rats. Sixty female Sprague-Dawley (SD) rats (aged 2-month-old) were randomly divided into six groups (n = 10), which were the sham control group (Group A), the model group (GroupB), the standard group (Group C), the treated 1 group (Group D), the treated 2 group (Group E), and the treated 3 group (Group F). Except the sham control group (Group A), rats in other groups had been ovariectomized. All rats were given the same feedstuff. Meanwhile, Group C was given calcium 75 mg·kg-1 combined with VitD3 21 IU·kg-1 by gastrogavage every day for 4 months; Group D was given EPF 75 mg·kg-1; Group E was given EPF 150 mg·kg-1;Group F was given EPF 300 mg·kg-1. At the end of the 4th month, all rats were sacrificed. Bones, which included tibia, femur and humerus of both sides and all lumbar vertebra bodies, had been taken out. Measurement was made on the elastic modulus, maximum loading capability, maximum stress, potential energy of deformation, and structural rigidity of biomechanical properties of the fourth lumbar vertebra body (LV4); the maximum loading capability, bone break load, potential energy of deformation, structural rigidity of the structural dynamics properties of the femur com-pact bone; the elastic modulus, maximum stress, maximum inherent strain, bone break stress, and bone break strain of the mechanical properties of a material of the femur compact bone in the experimental rats. The results showed that compared with Group B, the elastic modulus, maximum loading capability, maximum stress, potential energy of deformation, and structural rigidity of LV4; the maximum loading capability, bone break load, potential energy of de-formation, structural rigidity of the structural dynamics properties of the femur compact bone; the elastic modulus, maximum stress, maximum inherent strain, and bone break strain of the mechanical properties of a material of the fe-mur compact bone were obviously increased in Group A, D, E and F (P< 0.05). Group C had increasing tendency. There were no statistical differences among Group A, C, D, E and F. Group D, E, and F had increased with EPF dose-dependently. However, there were no statistical differences among them. There were no statistical differences on bone break strain of the mechanical properties of a material of the femur compact bone among Group A, C, D, E, and F. It showed that ovariectomization reduced the biomechanical properties of vertebra bodies, structural dynamics properties of the femur compact bone, and the mechanical properties of a material of the femur compact bone. The application of EPF can effectively prevent and treat the decreasing of biomechanical properties of ovariectomized rats, so as to keep them in a relatively higher level.