消痈溃得康及其拆方预防乙醇致大鼠急性胃黏膜损伤的抗氧化机制

摘要

目的:探讨消痈溃得康及其拆方对乙醇致大鼠急性胃黏膜损伤的保护作用及机制.方法:将48只Wistar大鼠随机分为正常组、模型组、消痈溃得康组、清热解毒组、和血止血组、托里生肌组.给药组连续给药5天,末次给药1h后,模型组和给药组灌胃1 mL无水乙醇,作用1h,造成大鼠急性胃黏膜损伤.以大体及光镜下观察评价消痈溃得康及其拆方对胃黏膜损伤的保护作用;采用生化检测法检测胃组织中谷胱甘肽(Glutathione,GSH)含量、脂质过氧化物(Lipid Peroxide,LPO)含量以及诱导型一氧化氮合酶(Inducible Nitric Oxide Synthase,iNOS)活力的变化.结果:消痈溃得康及各拆方组溃疡指数明显低于模型组(P＜0.01),给药组与模型组比较大鼠胃组织中GSH含量明显高于模型组(P＜0.01),LPO含量明显低于模型组(P＜0.01),iNOS酶活力明显低于模型组(P＜0.01).结论:消痈溃得康对乙醇诱导的大鼠急性胃损伤具有明显的保护作用,其中消痈溃得康组和清热解毒组药物作用最为明显,提示乙醇诱导的大鼠急性胃损伤符合中医毒热证证型,消痈溃得康及各拆方对其保护作用的机制可能与抗氧化有关.%Xiao Yong Kui De Kang (XYKDK) is a traditional Chinese medical (TCM) compound used for the treatment of gastric ulcer.This study was aimed at investigating the effects of its constituents from XYKDK on ethanol-induced gastric mucosal injury in rats and the mechanism behind its effect.Forty-eight Wistar rats (SPF grade) were randomly divided into 6 groups in accordance with TCM terminology and differentiation:the control group,the model group and Xiao Yong Kui De Kang group (XYKDK),Qing Re Jie Du group (QRJD),He Xue Zhi Xue group (HXZX) and Tuo Li Sheng Ji group (TLSJ).Medicinal-administration groups were pretreated with their corresponding relevant medicine for five days prior.After 1 h following the final of the last administration,the model group and varying administration groups were given 1 mL of ethanol to cause acute gastric mucosal injury in rats,while animals were euthanized 1 h after ethanol ingestion.The protective effects of XYKDK and its constituents were evaluated by calculating lesion indices and observing pathological changes.The contents of glutathione (GSH),lipid hydroperoxide (LPO) and inducible nitric oxide synthase (iNOS) in gastric mucosa were measured with biochemical assay to determine the protecting mechanism.As a result,it was found that the lesion indices of XYKDK and its constituents were significantly lower than those of the model group (P ＜ 0.01).The contents of GSH in the gastric mucosa of administration groups were higher,while LPO and iNOS were lower than that of the model group (P ＜ 0.01).In conclusion,XYKDK and its constituents showed an obvious protective effect on ethanol-induced gastric mucosal injury in rats,in which the XYKDK group and the QRJD group were better off than either of the other two groups.It was proved that the acute gastric injury induced by ethanol in rats was in conformity with the syndrome types found in TCM.The mechanism of action may be related to antioxidation.