目的研究乳铁蛋白多肽嵌合体对泛耐药铜绿假单胞菌生物被膜形成的作用。方法在泛耐药铜绿假单胞菌培养基（OD600=0.05）中分别加入乳铁蛋白多肽（LFcin、LFampin）以及乳铁蛋白多肽嵌合体（LFchimera）溶液干预；结晶紫法定量检测生物被膜；以结晶紫染色法，在光镜下观察生物被膜形态。结果乳铁蛋白多肽以及乳铁蛋白多肽嵌合体均能抑制泛耐药铜绿假单胞菌生物被膜形成（P<0.05），生物被膜量与多肽浓度呈负相关（P<0.05）；乳铁蛋白多肽嵌合体的作用强于乳铁蛋白多肽及其混合物（P<0.05)。结论乳铁蛋白多肽和乳铁蛋白多肽嵌合体，尤其是LFchimera对泛耐药铜绿假单胞菌生物被膜形成具有显著的抑制作用，有望用于泛耐药铜绿假单胞菌感染的治疗。%Objective To study the effect of LFchimera on the biofilm formation in pandrug-resistant pseudomonas aeruginosa. Methods The solution of LFcin and LFampin, LFchimera were added into the culture medium of pandrug-resis-tant pseudomonas aeruginosa to evaluate the effect on biofilm formation respectively; biofilm quantification assay was performed by staining with crystal violet and spectrophotometer assay; biofilm morphology was viewed by optical microscopy after being stained with crystal violet. Results LFcin and LFampin, LFchimera could both inhibit the biofilm formation of pandrug-resis-tant pseudomonas aeruginosa, and the effect was positively correlated with their concentrations (P<0.05). The effect of LFchimera was greater significantly than LFcin, LFampin and their mixtures (P<0.05). Conclusion LFcin, LFampin and LFchimera, especially LFchimera could inhibit the biofilm formation in pandrug-resistant pseudomonas aeruginosa, it is promis-ing to be a new compound for the treatment for pandrug-resistant pseudomonas aeruginosa infections.
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