中药白花蛇舌草提取物对人体癌细胞及小鼠实体瘤的抑制作用研究

摘要

Objective It is to explore the inhibitory action of herb Hedyotis Diffusae ( TCM) extract HD-1 on human cancer cells and solid tumor in mice .Methods MTT assay was used in the vitro experiments on human cancer cells .Growth inhibition of herb Hedyotis Diffusae extract HD -1 was analyzed in HepG2 cells and human colorectal cancer cell lines HCTI 16.Results of weight of tumor -bearing mice, tumor inhibition rate, thymus index and spleen index were compared on S 180 solid tumor in vivo mouse model between high -dose group and low dose group .Results In vitro experiments , inhibition rate of HD-1 extract on HepG2 and HCTI 16 were related to drug concentrations and drug effect time .In vivo experiments , the high HD-1group and low -HD-1 group tumor weight were reduced compared with the model group .Inhibition rates were 13.28%, 26.47%respectively.Spleen index in the high HD -1 group and low-HD-1 group was significantly higher than that in the model group (P<0.05).In the CTX group and CTX+HD-1 group, thymus index and spleen index , liver index, lung index were statistically significant (P<0.05).Concl usion Herba hedyotis diffusae (TCM) extract could inhibit HepG2 cells and human colon cancer cell lines HCTI 16 straight growth, and inhibit the growth of solid tumors in mice .%目的：探讨中药白花蛇舌草提取物（ HD-1）对人体癌细胞及小鼠实体瘤的抑制作用。方法对人体癌细胞采用 MTT法进行体外实验，检测白花蛇舌草提取物HD-1对人肝癌细胞HepG2和人直结肠癌细胞株 HCTI 16生长的抑制作用；对S180实体瘤小鼠模型进行体内实验，对比高、低剂量的白花蛇舌草提取物HD-1对荷瘤小鼠体质量、抑瘤率、胸腺指数、脾指数等指标的影响。结果体外实验显示，中药白花蛇舌草提取物对HepG2和HCTI 16的抑制率和给药浓度、药物作用时间相关。体内实验显示，与模型对照组相比，高HD-1组、低HD-1组瘤质量均减轻，抑制率分别为13．28％，26．47％，脾脏指数明显升高（ P＜0.05）。 CTX组与CTX＋HD-1组比较，小鼠胸腺指数、脾脏指数、肝脏指数、肺脏指数均有显著性差异（ P均＜0．05）。结论中药白花蛇舌草提取物体外能够明显抑制人肝癌细胞HepG2和人直结肠癌细胞株HCTI 16的生长，能够明显抑制小鼠体内实体瘤的生长。