枫蓼肠胃康分散片联合泮托拉唑对乙醇损伤的人胃黏膜上皮GES-1细胞的作用研究

摘要

Objective It is to investigate the protective effect of Fengliaochangweikang dispersible tablets with pantoprazole on human gastric mucosal epithelium GES-1 cells damaged by ethanol.Methods The GES-1 cell samples cultured in vitro were selected and were cultured in 4％～8％ ethanol culture solution for 2～5 h to establish stable ethanol damaged gastric mucosa GES-1 cell models.The damaged cells were divided into Fengliaochangweikang dispersible tablets with pantoprazole group (combination group) and pantoprazole group and were treated with respective medicine for 24h in 100 uL medium,and their cell activity was determined by MTT method.Results After the GES-1 cells was cultured in 7％ ethanol medium for 3 h,the cell inhibition rate was 40.00％,and they were the best gastric mucosal epithelium models.The cell absorbance value under different medicine concentration was significantly higher in the combination group than that in the pazopazole group (P ＜0.05).Conclusion Fengliaochangweikang dispersible tablets with pantoprazole can protect the damaged GES-1 cells at lower dose,and the combined application is better than that of pantoprazole used singly,they are worthy of popularization and application.%目的 探讨枫蓼肠胃康分散片联合泮托拉唑对乙醇损伤的人胃黏膜上皮GES-1细胞的保护作用.方法 选取体外培养的GES-1细胞样本,以浓度为4％～8％的乙醇培养液培养2～5h,建立稳定的乙醇损伤胃黏膜GES-1细胞模型.将损伤细胞分为枫蓼肠胃康分散片联合泮托拉唑组(联合组)和泮托拉唑组,在100μL含有不同浓度药物的培养基中加入联合组和泮托拉唑组的GES-1细胞,持续培养24 h,利用MTT方法测定细胞活性.结果 GES-1细胞于7％乙醇培养液中培养3h后,细胞抑制率为40.00％,为最佳胃黏膜损伤模型;联合组不同药物浓度下细胞吸光度值均明显高于泮托拉唑组(P均＜0.05).结论 枫蓼肠胃康分散片联合泮托拉唑在较低剂量时就可以保护受损GES-1细胞,联合应用较泮托拉唑单用效果好,值得临床推广应用.