血清miR-21和miR-10b在早期宫颈癌患者中的表达及临床意义

摘要

目的 观察血清miR-21和miR-10b在早期宫颈癌患者中的表达及临床意义.方法 选择手术治疗的宫颈上皮内瘤样变(CIN)患者45例作为CIN组,早期宫颈浸润癌(ICC)患者85例作为ICC组,子宫肌瘤全切子宫后正常宫颈上皮(NCE)患者20例作为NCE组.用Real time PCR法检测各组血清miR-21和miR-10b表达情况及ICC组手术后miR-21和miR-10b表达情况,分析miR-21和miR-10b相对表达量与CIN 分级、ICC临床指标的关系及ICC患者血清miR-21和miR-10b表达的相关性.结果 ICC组的miR-21相对表达量明显高于CIN组和NCE组(P均<0.05),并随着CIN分级的升高相对表达量增加(P<0.05),手术后血清miR-21相对表达量明显降低(P<0.05);ICC组的miR-10b相对表达量明显低于CIN组和NCE组(P均<0.05),并随着CIN分级的升高相对表达量降低(P<0.05),手术后血清miR-10b表达量明显增加(P<0.05).血清miR-21和miR-10b表达量与年龄、组织类型、肿瘤直径、肌层浸润深度、脉管浸润和神经浸润无明显相关性(P均>0.05),而与临床分期、组织分化程度、高位HPV和淋巴转移具有明显的相关性(P均<0.05).ICC患者的血清miR-21相对表达量与血清miR-10b相对表达量呈负相关(r=-0.618,P<0.05).结论 miR-21可能为促癌基因,miR-10b为抑癌基因,miR-21和miR-10b参与了宫颈癌的发生发展,联合检测血清miR-21和miR-10b表达量对于早期诊断宫颈癌和监测预后具有重要意义.%Objective It is to observe the expression of serum miR-21 and miR-10b and their clinical significance in patients with early cervical cancer.Methods 45 patients with cervical intraepithelial neoplasia(CIN) and 85 patients with early invasive cervical cancer (ICC) were selected after surgery as CIN group and ICC group respectively,and 20 patients with normal cervical epithelium (NCE) were also selected after total resection of uterine fibroids as NCE group.The serum miR-21 and miR-10b expression were detected by Real time PCR,the expression levels of miR-21 and miR-10b were observed in NCE,CIN and ICC groups,the miR-21 and miR-10b levels were also observed in different CIN grades,the relationship among the levels of those in ICC,clinical indicators,their impacts before and after surgery and their correlation were observed too.Results The relative expression levels of miR-21 in ICC group was higher than those in CIN and NCE group (P<0.05),the levels of miR-21 in ICC group increased as the CIN grade increased (P<0.05),and the levels of those decreased after surgery (P<0.05);the relative expression of miR-10b in ICC group was significantly lower compared with CIN and NCE group (P<0.05),the levels of those decreased as CIN grade increased,and the levels of those increased after surgery.There was no significant correlations among the levels of serum miR-21 and miR-10b expression,age,histological type,tumor size,depth of myometrial invasion,vascular invasion,and perineural invasion (P>0.05),but there were significant correlations among the levels of those,clinical stage,histological grade,high HPV and lymph node metastasis (P<0.05).The serum relative expression levels of miR-21 were negatively correlated to the miR-10b in ICC patients (r=-0.618,P<0.05).Conclusion MiR-21 may be cancer-promoting gene and miR-10b may be as a tumor suppressor gene,miR-21 and miR-10b involved in the formation process of the ICC,it is important for the early diagnosis and monitoring prognosis of ICC in detection of serum miR-21 and miR-10b expression.