珍珠水解液的美白作用机理研究

摘要

目的:通过人的黑色素细胞实验研究珍珠水解液的美白作用机理.方法:NaOH法测定黑色素细胞黑色素含量,实时荧光定量PCR法(RT-PCR)测定珍珠水解液对影响黑色素合成的相关基因酪氨酸酶基因(TYR)、酪氨酸酶相关蛋白1 (TRP1)及影响黑色素转运的相关基因小眼畸形相关转录因子(MITF).结果:浓度为0.25％、0.5％、0.75％的珍珠水解液可以抑制黑色素细胞的黑色素合成,3种浓度下的珍珠水解液均可抑制相关基因TRP1mRNA的表达,0.25％浓度下可抑制TYR、TRP1及MITF的mRNA的表达.结论:珍珠水解液主要通过抑制黑色素合成过程中TRP1 mRNA的表达,从而抑制黑色素合成而发挥美白功效.%Objective:To study skin-whitening mechanism of Margarita liquid by in vitro melanoma cells tests.Methods:The skin-whitening activities were evaluated by measuring human melanocytes melanin synthesis inhibition activity,and Real-time PCR (RT-PCR)method was further used to evaluate the expression of microphthalmia-associated transcription factor (MITF),tyrosinase-related protein 1 (TRP1) and Tyrosinase(TYR)mRNA.Results:0.25％,0.5％ and 0.75％ concentration of Margarita liquid could significantly decreased human melanocytes melanin synthesis,all the three concentrations of Margarita liquid could decrease the expression of TRP1 mRNA,and 0.25％ concentration of Margarita liquid could decrease the expression of TYR、TRP1 and MITF mRNA.Conclusion:Margarita liquid was detected to have skin-whitening activity through in vitro Human melanoma cells tests,it could be used as a skin-whitening cosmetic additive.