大叶蛇葡萄提取物对SMMC-7721细胞增殖抑制作用的研究

摘要

Objective:To explore the inhibitory effect of leaves and tender stem extracts of Ampelopsis megalophylla on SMMC-7721 cells proliferation and screen the anti-tumor active ingredients in vitro.Methods:Petroleum ether,ethyl acetate (EtOAc) and aqueous extracts of A.megalophylla as well as five compounds,ampelopsin,myricetin,myricetrin,emodin and quercetin were selected to treat SMMC-7712 at different concentration for 24,48,72 h through MTT assay,respectively.Results:Treatment with EtOAc extract or emodine inhibited the cell proliferation in different degrees.EtOAc extract and emodin could inhibit cell proliferation in a time-effect relationship in 100 μg· mL-1 and in 40,80 and 160 μmol· L-1,respectively.IC50 of EtOAc extract and emodin on SMMC-7721 cells in 72 h were 110.81 μg· mL-1 and 53.20 μmol· L-1.Conclusion:Extracts of A.megalophylla has good anti-tumor activity in vitro.%目的:研究大叶蛇葡萄叶及嫩茎提取物对SMMC-7721细胞增殖的影响,筛选其有效抗肿瘤活性物质.方法:采用MTF法检测不同质量浓度的大叶蛇葡萄石油醚萃取物部位、乙酸乙酯萃取物部位及水溶性物部位,以及该植物中含有的蛇葡萄素、杨梅素、杨梅苷、大黄素及槲皮素分别作用24、48、72 h后对SMMC-7721细胞增殖的抑制作用.结果:乙酸乙酯萃取物部位和大黄素均能不同程度地抑制SMMC-7721细胞增殖,乙酸乙酯萃取物部位对细胞的抑制作用在100 μg· mL-1时具有一定的时效关系,大黄素对细胞的抑制作用在40、80、160 μmol·L-1时也具有一定的时效关系,乙酸乙酯萃取物部位和大黄素作用SMMC-7721细胞72 h的IC50分别为110.81 μg·mL-1和53.20 μmol·L-1.结论:大叶蛇葡萄提取物具有较好的体外抗肿瘤活性.