以残败花梗为外植体进行组织培养,建立了蝴蝶兰的无菌繁殖体系,研究了培养基中不同种类植物生长调节物质及其浓度比例对休眠芽的萌发、丛生芽的诱导以及试管苗生根的影响,筛选出了最佳培养基组成;研究了活性炭、PVP、温度、暗培养对培养基褐化的影响。结果表明：在1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10%香蕉汁＋3%蔗糖＋0.6%琼脂的培养基上休眠芽的诱导效果最好,诱导率可达87.5%;在1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10%香蕉汁＋3%蔗糖＋0.6%琼脂的培养基上丛生芽的诱导效果最好,增殖系数可达3.15;在1/2 MS＋NAA 0.5 mg/L＋活性炭1 000 mg/L＋3%蔗糖＋0.6%琼脂的培养基上生根效果最好,生根率可达95%,平均每株生根3.11条左右,炼苗后移植成活率可达90%。%Flower peduncles of Phalaenopsis amabilis（L.）C.L.Blume were cultured to induce seedlings on medium,then shoots were cultured as explants to produce shoot cluters in vitro.The effects of different plant growth regulators and their concentrations on the germination of dormant buds,induction of clusters buds and vitro rooting were studied to select the best medium.The effects of activated charcoal,PVP,temperature,dark culture on the medium browning were also investigated.The results showed that：the best media inducing dormant buds was 1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10% banana juice＋3% sucrose＋0.6% agar,the induction rate of shoots was up to 87.5%.The best media inducing clusters buds was 1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10% banana juice＋3% sucrose＋0.6% agar,the multiplication coefficient was up to 3.15.The best media inducing rooting was 1/2 MS＋NAA 0.5 mg/L＋1 000 mg/L activaed charcoal＋3% sucrose＋0.6% agar,the rooting rate reached to 95%,average number of roots was about 3.11 stripes/plant,transplant survival rate was 90% after hardening seedling.
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