草莓细菌叶斑病病原鉴定

摘要

Traditional bacterial identification measures by appearance observation and physiology bio-chemical tests and the mordern bacterial identification measures by 16S rDNA technique were used to identify the bacterial pathogen of leaf spot on strawberry. The results showed that the strain could use glucose,lactose,maltose,sucrose,D-Trehalose,starch,mannitol,inositol,L-Valine and L-Proline. The strain was positive for nitrate reduction, arginine cihydrolase and oxidase reaction,but negative for fat hydrolysis,starch hydrolysis and gelatin hydrolysis. At last,the strain was identified as Xanthomonas fragariae.%采用形态观察、生理生化特性测试等传统的细菌鉴定方法和16srDNA碱基序列测定等现代的细菌鉴定方法鉴定草莓细菌叶斑病菌株。结果表明：该菌株可利用的碳源有葡萄糖、乳糖、麦芽糖、蔗糖、D-海藻糖、淀粉、甘露醇、肌醇、L-缬氨酸、L-精氨酸。硝酸盐还原试验为阳性，淀粉水解阴性，明胶水解阴性，油脂水解阴性，精氨酸双水解酶阳性，接触酶阳性；鉴定该菌株为草莓黄单孢菌（Xanthomonas fragariae）。