粤西地区5种荔枝nrDNA ITS基因扩增及酶切分析研究

摘要

The PCR amplifications of ITS in Ribosomal DNA(rDNA)of five kinds of litchis grown in Guangdong Province were researched,using restriction enzyme HhaI,HaeⅢ,EcoR Ⅰ,HindⅢ,RsaⅠinto the enzyme digestion analysis.All these five kinds of litchis’ ITS genetic sequence lengths were 600~800 bp.After the enzyme digestion of PCR amplification,five kinds of litchis’ PCR amplifications were digested in the position of main belt between 600~800 bp.The litchis of Feizixiao were different from all other four spices in the main belt.The research results also revealed that the litchis of Baitangying,Xinxing,Nuomici and Shangshuhuai were relatively closed in the aspect of affinity,while the litchis of Feizixiao were more distant.%对广东省粤西地区的5个荔枝品种进行核糖体DNA(rDNA)内转录间隔区(ITS)的PCR扩增，并用限制酶HhaI、Hae芋、EcoR玉、Hind芋、Rsa玉对扩增产物进行酶切分析。5种荔枝属植物的ITS基因序列长度为600~800 bp，PCR产物经过5种酶切后，5种荔枝属植物的PCR产物均切出位于600~800 bp之间的主带，妃子笑的主带与其余4种差异明显；结果表明，荔枝品种中的白糖罂、新兴、糯米糍、尚书怀4种的亲缘性比较近，妃子笑与这4种的亲缘性较远。