Objective To study the combined effect of extremely low frequency electromagnetic fields ( ELF-EMF ) exposure (50 Hz and 3 mT) and high temperature exposure on hsp22 and hsp26 expression in Drosophila melanogaster. Methods Under the conditions of 50 Hz and 3 mT ELF-EMF,the male and female D.melanogaster was separately exposed for 2 h at 25℃, 27.5℃, 30℃, 32.5℃,and 35℃, respectively.The death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster were detected after exposure;or at 25℃and 35℃at 2 h, 4 h, 8 h, and 24 h, respectively.The hsp22 and hsp26 expression levels of D.melanogaster were tested after exposure .Results After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, temperature had a significant effect (P<0.01) on the death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster, but ELF-EMF had no significant effect (P>0.05).After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, the hsp22 and hsp26 expression levels of D.melanogaster were significantly impacted(P<0.01) by gender, temperature, ELF-EMF and exposure time, and tem-perature was the most important factor .Gender, temperature, ELF-EMF and exposure time had some interaction with the results.Conclusion ELF-EMF can influence the expression levels of hsp22 and hsp26.ELF-EMF exposure contributes to heat tolerance in D.melanogaster through accelerated expression of hsp22 and hsp26.%目的：探讨在高温（35℃）条件下，极低频电磁场（ extremely low frequency electromagnetic fields ，ELF-EMF）对雌雄黑腹果蝇hsp22和hsp26基因表达水平的影响。方法在25、27．5、30、32．5、35℃下，1日龄雌、雄黑腹果蝇在50 Hz、3 mT ELF-EMF暴露2 h，测定其死亡率、运动能力和hsp22、hsp26表达水平；在25和35℃下，1日龄雌、雄黑腹果蝇在50 Hz、3 mT ELF-EMF暴露2、4、8、24 h，测定其hsp22、hsp26表达水平。结果在25、27．5、30、32．5、35℃下暴露2 h，温度变化对果蝇的死亡率、运动能力和hsp22、hsp26表达水平有极显著影响（P＜0．01），ELF-EMF对hsp22、hsp26表达水平无显著影响（ P＞0．05）；在25和35℃下分别暴露2、4、8、24 h，性别、温度、ELF-EMF暴露和暴露时间对hsp22、hsp26的表达水平有极显著影响，其中，温度是主要的影响因素，且若干因素间有交互作用。结论 ELF-EMF可影响果蝇hsp22、hsp26的表达，且ELF-EMF可能具有降低因高温环境所致负面影响的作用，提高果蝇对高温环境的耐受能力，缓解高温环境对果蝇的损伤效应。
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