大红袍中一个新的香豆素和八个已知酚酸类化合物

摘要

Objective To investigate the chemical constituents of the Radix Campylotropidis Hirtellae ( RCH ) . Methods 60%ethanol extract of the RCH was separated by column chromatography on silica gel, ODS and Sephadex LH-20 to obtain aimed compounds.Structures of the compounds were investigated by modern spectroscopy.Results Nine compounds were separated from Campylotropis hirtella ( Franch.) Schindl, and the structure of the new compound 1 was determined by spectral analysis and named hedysarimcoumestanⅠ( 1 ) , while the others were identified as the known isotrifoliol(2), hedysarimcoumestan B(3), umbelliferone(4), orobol(5), quercetin(6),(+) catechin(7), kaempferol (8) and dihydrokaempferol(9) .Conclusion Compound 1 is a new compound and 3-5 are separated from C.hirtella for the first time.The screening results of inhibition of H5N1 virus activity show that compound 5 has some inhibitory activity at a level of 50.0 μg /ml.%目的：研究大红袍（ RCH）的化学成分。方法利用重结晶、硅胶、ODS、Sephadex LH-20等色谱技术对大红袍60％乙醇提取物进行分离并纯化得到化合物，采用现代波谱技术阐明化合物结构。结果分离得到9个化合物，其中，1个新化合物1的结构根据波谱解析结果予以确定并命名为红花岩黄芪香豆雌酚Ⅰ（1），而8个已知化合物则分别鉴定为异槲皮酚（isotrifoliol，2）、红花岩黄芪香豆雌酚B（3）、伞形花内酯（4）、香豌豆酚（5）、槲皮素（6）、（＋）儿茶素（7）、山奈酚（8）和二氢山奈酚（9）。结论化合物1为新化合物，化合物3～5首次从该植物中分离得到。抗H5N1病毒细胞活性筛选结果显示，化合物5在50．0μg／ml水平对H5N1有一定的抑制活性。