目的：探究减数分裂重组蛋白11同系物A（MRE11）在不同刺激物诱导下炎症小体激活中的作用和地位。方法利用不同刺激物，如Poly（I∶C）、Poly（dA∶dT）、E．coli gDNA、293T gDNA和CPPD以及生殖疱疹病毒（HSV）等处理单核巨噬细胞THP-1，通过IL-1β酶联免疫吸附试剂盒（ ELISA）筛选出诱导激活炎症小体的有效刺激物；合成siMRE11干扰小RNA，转染THP-1细胞，免疫印迹检测MRE11干扰下调效率；利用筛选到的阳性刺激物处理MRE11干扰下调的细胞，采用ELISA和免疫印迹方法检测MRE11对细胞分泌炎性因子IL-1β水平和前胱天蛋白酶（pro-caspase）-1切割的影响。结果在MRE11干扰下调的THP-1细胞中，Poly（I∶C）、Poly（dA∶dT）、E．coli gD-NA和293 T gDNA 刺激激活细胞分泌的IL-1β水平以及前胱天蛋白酶-1切割的水平均有不同程度降低。结论MRE11参与由DNA以及RNA刺激激活的炎症小体信号通路。%Objective To evaluate the function of MRE11 in inflammasome activation.Methods Different stimuli,in-cluding Poly(I∶C), Poly(dA∶dT),E.coli gDNA,293T gDNA,CPPD and HSV,were used to identify the effective inflamma-some activator using ELISA.Then, MRE11 siRNA oligos were sythesized and transfected into THP-1 cells while Western blotting was used to analyze the efficacy of MRE 11 knockdown .Finally ELISA and Western blotting were used to analyze the involvement of MRE11 in inflammasome activation induced by Poly (I∶C), Poly(dA∶dT), E.coli gDNA and 293T gDNA. Results The IL-1βsecretion and pro-caspase-1 activation which induced by Poly ( I∶C) , Poly( dA∶dT) , E.coli gDNA and 293T gDNA were reduced with different degrees in MRE 11-knockdown THP-1 cells.Conclusion These results indicate that MRE11 is required for inflammasome activation induced by genetic materials .
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