阿特拉津降解菌株的分离和鉴定

摘要

从农药厂废水中分离到6株能以除草剂阿特拉津为唯一氮源生长的细菌，即假单胞菌(Pseu-domonas spp.)AD1、AD2和AD6，土壤杆菌(Agrobacterium sp.)AD4，黄单胞菌(Xanthomonas sp.)AD5，欧文氏菌(Erwinia sp.)AD7。AD1菌株能使无机盐培养基中的0.3g/L阿特拉津在72h内降解99.9％。当以AD1、AD2、AD4、AD5、AD6和AD7菌株的总DNA为模板进行PCR扩增时,除AD2菌株以外，均得到了与文献报道的假单胞菌ADP菌株的阿特拉津氯水解酶基因(atzA)同源的PCR产物。%Six atrazine-degrading strains, Pseudomonas spp. AD1, AD2, AD6, Agrobacterium sp. AD4, Xanthomonas AD5, and Erwinia sp. AD7, were isolated from industrial wastewater. These strains are able to grow on atrazine as sole nitrogen source. Strain AD1 is able to degrade atrazine of 0. 3g/L in minimal medium at a percentage of 99.9% in 72 hours. PCR products that are homologous to the atrazine chlorohydrolase gene atzA)from Pseudomonas sp. strain ADP were obtained by PCR method using total DNA of the strains AD1 ,AD4,AD5,AD6,and AD7 as templates.