长双歧杆菌BBMN68可溶性蛋白质图谱的建立

摘要

为了建立长双歧杆菌BBMN68蛋白质图谱,采用双向电泳的方法建立了2-D参考图谱.通过MALDI-TOF/MS质谱鉴定和数据库搜索,鉴定到206个蛋白质(占长双歧杆菌BBMN68基因预测总蛋白的11.4%).通过2-D胶分析,共有800±15(对数期)和800±20(稳定期)个蛋白质,其中282个蛋白点成功鉴定,代表206个不同的蛋白质.另外,分析了实验鉴定蛋白质的等电点和分子量,蛋白功能,密码子偏好性,蛋白质疏水性以及蛋白质细胞定位的分析.研究结果为长双歧杆菌的比较蛋白质组学研究提供了参考图谱和蛋白质基础信息数据.%To establish proteomic maps of Bifidobacterium longum subsp. longum BBMN68, applying two-dimensional gel electrophoresis, we built a 2-D reference map, by MALDI-TOF mass spectrometry.We identified a total number of 206 different proteins (11.4％ of predicted total proteins in B. longum subsp. longum BBMN68). A total of 800±15 (exponential) and 800±20 (stationary) protein spots on each 2D-PAGE map were got, in which a total of 282 spots were successfully identified, represented 206 different proteins. Additionally, combined with analysis of pl and molecular masses, COG, CAI,GRVAVY and the cellular localizations of identified proteins in B. longum subsp. longum BBMN68.This study presented a proteome map of B. longum BBMN68 and provided a reference proteome database for comparative proteomic studied.